Abstract: Corrosion induced by the microorganisms is a phenomenon still poorly known and difficult to interpret. In this work we are interested in marine corrosion of stainless steel 254 SMO by Amazonians biofilms. The objective is to achieve an experimental laboratory to reproduce the corrosion completely controlled environment. The microbiologically influenced corrosion (MIC) of stainless steel by marine bacteria was investigated using surface analysis (scanning electron microscopy (SEM)), and electrochemical techniques (the open circuit potential, electrochemical impedance spectroscopy (EIS), and potentiodynamic polarization curves). The epifluorescence microscopy has been used to correlate the experiments. We investigated the role of some chemical parameters and the influence of the tide.